marta lwin
contact marta (at) metabreed (dot) com
ITP masters thesis 2006
lab2 documentation : DNA amplification
Special thanks to Rebecca Budinoff and Warren Weaver.
from the
Comparative Genetics Laboratory American Museum of Natural History
DNA amplification:
the previously extracted DNA is prepared for PCR amplification
the small white balls in the tubes create the necessary environment for the amplification.
first add pure water
primers targeting specific sections of the gene, where it is know to show unique species information
QT movie of the process: primers (18MB)
the DNA goes in last to prevent any contamination of primers
amplification machines, heat and cool the mixture the get the primers to adhere to the dna strand in the correct area of the gene.
next step
DNA sequencing
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