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marta lwin
contact marta (at) metabreed (dot) com ITP masters thesis 2006 lab3 documentation : DNA sequencing Special thanks to Rebecca Budinoff and Warren Weaver. from the Comparative Genetics Laboratory American Museum of Natural History DNA sequencing: 
After the primers are added to the DNA, and amplified, the DNA is tested by creating a gel. This stage is to confirm that you have DNA, it is a check point in the process. 
This gel is created by dying the DNA and passing electricity through it, to create a ban of colors. 
This robot was designed to automate the otherwise lengthy process of cleaning the DNA of extra proteins, and primers and nucleotides, which would otherwise "pollute" your sequence. movie of the process: coming soon 
This is the machine where the sequencing takes place. Electricity is passed through a thin wire piercing the DNA, which makes the proteins move. The rate of motion is different for each protein,which then enables the machine to take an image of each protein as it moves. Eventually creating a photo representation of the number of A,T,C,G in the sequence. 
The is what the array looks like. This one has 96 wires, able to sequence that number of samples at once. 
This is the photograph of our sequence, which took a few hours. 
This is the photograph of our sequence in text format. The complete cycle of going from body to binary. |
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